SARS-COV-2 RT-qPCR Assay (Triple Fluorescence RT-PCR Method)

The SARS-COV-2 RT qPCR Assay is a real time (rt) reverse transcriptase (RT) polymerase chain reaction (PCR) test intended for the qualitative detection of ORF1ab and N genes of the SARS CoV 2 in nasopharyngeal ( NP) swab, oropharyngeal (OP) swab and sputum specimen collected by a healthcare worker, from patients suspected of COVID-19 by their health care provider.

Features

High Accuracy

Limit of Detection: 200 copies/mL 

Sensitivity: 100 % 

Specificity: 99.1% 

Accuracy: 99.3%


Convenient Operation

Triplex-detections in a single tube (ORF1ab & N genes, human RNAse P (RNP) gene) 

Primer and probe premixed liquid form


Reliable

Anti-pollution system: UDG 

Internal control: Human RNaseP (RNP) gene  

Positive control: Pseudovirus


Fast

Result in about 1 hour


APPLICABLE INSTRUMENTS:

Real-time PCR instrument with FAM, TEXAS RED/ROX and HEX/VIC channels, such as ABI7500, ABI Q3, ABI Q6, Bio Rad CFX96.


Specification

Product features Parameters
PrincipleTriple Fluorescence RT-PCR
FormatTube
SpecimenNasopharyngeal Swab /Oropharyngeal Swab / Sputum
CertificateCE
Reading Time1 hour
Pack50T /100T
Storage Temperature-30~15°C
Shelf Life6 Months
Sensitivity100%
Specificity99.1%
Accuracy99.3%
Cut-Off200 copies/mL

Ordering Information

Cat. No. Product Specimen PackCut-off